Can you isolate adipocytes from WAT for culture?
Yes, it is possible to isolate adipose tissue explants and culture them.
Nice study Shripa – how does GR expression change in obesity and does it depend on the fat location/type?
Thank you Dr. Stokes. Based on literature, the relationship between obesity and GR expression is not entirely clear but a number of animal and human studies have shown a decreased expression of GR with obesity. GR expression depend on the location of fat. For example when there is increased level of Glucocorticoids (e.g. Cushing’s syndrome), there is increase in accumulation of visceral fat and decrease in subcutaneous fat.